Table 1. Summary of Ty1 replication data for NPC mutant yeast strains.
| NPC substructure | Yeast strain | Ty1 mobility frequency (SD) | Ty1 mobility ratioa | SUF16b | SUP61b | Ty1 mRNAc | Ty1 Gag protein levelsd | Ty1 cDNAe | tLEUf | tLEU (denovo)f | tGLYf |
|---|---|---|---|---|---|---|---|---|---|---|---|
| − | wild type | 6.80E−06 (3E−06) | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.2 | 1.0 | 1.0 | 1.0 |
| Outer ring | nup84Δ | 5.37E−06 (2E−06) | 0.79 | 0.5 | 0.09 | − | − | − | − | − | − |
| nup120Δ | 2.40E−05 (1E−05) | 3.53 | 9.8 | 2.4 | 1.6 | 1.1 | 8.3 | 0.7 | 0.7 | 0.8 | |
| nup133Δ | 4.17E−06 (1E−06) | 0.61 | 0.7 | 1.6 | − | − | − | − | − | − | |
| sec13-1 | 8.28E−07 (3E−07) | 0.12 | 0.05 | 0.07 | 1.2 | 0.84 | 0.4 | 1.0 | 0.8 | 0.7 | |
| Inner ring | nup170Δ | 4.00E−07 (3E−07) | 0.06 | 0.19 | 0.0 | 1.1 | 0.53 | 0.6 | 0.5 | 0.6 | 0.6 |
| nup188Δ | 6.22E−07 (9.9E−07) | 0.09 | 0.07 | 0.02 | 2.2 | 0.4 | 2.1 | 0.5 | 0.6 | 0.7 | |
| nup53Δ | 2.34E−06 (7E−07) | 0.34 | 0.08 | 0.09 | 1.2 | 1.1 | 2.9 | 0.7 | 0.9 | 0.7 | |
| nup59Δ | 5.26E−06 (5E−06) | 0.77 | 0.21 | 0.16 | − | − | − | − | − | − | |
| nup192-15 | 5.44E−07 (3E−07) | 0.08 | 0.0 | 0.09 | 2.2 | 0.73 | 1.0 | 1.1 | 0.9 | 1.2 | |
| Cytoplasmic | nup159-1 | 1.12E−07 (5E−08) | 0.02 | 0.05 | 0.003 | 0.52 | 0.37 | 0.5 | 1.0 | 1.2 | 1.4 |
| nup42Δ | 8.09E−06 (2E−06) | 1.19 | 0.02 | 0.04 | − | − | − | − | − | − | |
| Transmembrane ring | ndc1–4 | 6.23E−06 (3E−06) | 0.92 | 0.04 | 0.3 | − | − | − | − | − | − |
| pom34Δ | 1.70E−06 (1E−06) | 0.25 | 0.0 | 0.06 | 1.5 | 1.2 | 1.3 | 0.9 | 1.7 | 1.6 | |
| pom152Δ | 4.89E−06 (2E−06) | 0.72 | 0.21 | 0.27 | − | − | − | − | − | − | |
| Central FG | nup100Δ | 4.55E−06 (2E−06) | 0.67 | 0.16 | 0.07 | − | − | − | − | − | − |
| Nuclear | nup2Δ | 8.07E−06 (6E−06) | 1.19 | 0.21 | 0.25 | 1.6 | − | − | 0.51 | 0.44 | 0.62 |
| nup60Δ | 2.18E−06 (2E−06) | 0.32 | 0.0 | 0.03 | 0.19 | 0.23 | 0.2 | 0.5 | 0.6 | 0.5 | |
| mlp1Δ | 2.34E−06 (2E−06) | 0.34 | 0.83 | 0.2 | 0.79 | 0.77 | 0.5 | 0.5 | 0.6 | 0.8 | |
| mlp2Δ | 1.53E−05 (1E−05) | 2.25 | 2.1 | 0.63 | 1.3 | 0.75 | 1.1 | 0.6 | 0.6 | 0.8 | |
| nup1Δ | 6.84E−06 (5E−06) | 1.0 | 0.12 | − | 1.65 | 0.81 | − | 0.4 | 0.78 | 0.91 |
aCompared to wild type Ty1 mobility.
bCompared to wild type Ty1 insertion upstream of the SUF16 or SUP61 loci.
cCompared to wild type Ty1 mRNA levels with TAF10 as an internal control.
dCompared to wild type Ty1 Gag protein levels normalized to a GAPDH loading control.
eCompared to ratio of Ty1 cDNA to an endogenous Ty1 element.
fCompared to wild type tRNA levels with TAF10 as an internal control.
Standard deviation (SD), experiments not done indicated with a dash.