Table 1. Bacterial strains, media, plasmids and primers used in this study.
| Strains | Details/reference | Plasmids used for construction (if required) |
|---|---|---|
| ZAP193 | E. coli O157:H7 stx-, NCTC 12900 | |
| Sakai stx- | Dahan et al. (60) | |
| Sakai stx- Hfq-HTF | Tree et al. (31) | |
| ZAP1419 | ZAP193: micF- | pDW-mFft then pDW-mFf2 |
| ZAP1420 | ZAP193: micA- | pDW-mAft then pDW-mAf2 |
| ZAP1421 | ZAP193: rybB- | pDW-bBft then pDW-bBf2 |
| ZAP1422 | ZAP193: ryhB- | pDW-hBft then pDW-hBf2 |
| ZAP1423 | ZAP193: omrA- | pDW-oAft then pDW-oAf2 |
| ZAP1424 | ZAP193: omrB- | pDW-oBft then pDW-oBf2 |
| ZAP1425 | ZAP193: spf- | pDW-sfft then pDW-sff2 |
| ZAP1426 | ZAP193: fnrS- | pDW-fSft then pDW-fSf2 |
| ZAP1427 | ZAP193: mcaS- | pDW-mSft then pDW-mSf2 |
| ZAP1428 | ZAP193: rprA- | pDW-rAft then pDW-rAf2 |
| ZAP1429 | Sakai: stx-, micF- | pDW-mFft then pDW-mFf2 |
| ZAP1430 | Sakai: stx-, micA- | pDW-mAft then pDW-mAf2 |
| ZAP1431 | Sakai: stx-, rybB- | pDW-bBft then pDW-bBf2 |
| ZAP1432 | Sakai: stx-, ryhB- | pDW-hBft then pDW-hBf2 |
| ZAP1433 | Sakai: stx-, omrA- | pDW-oAft then pDW-oAf2 |
| ZAP1434 | Sakai: stx-, omrB- | pDW-oBft then pDW-oBf2 |
| ZAP1435 | Sakai: stx-, spf- | pDW-sfft then pDW-sff2 |
| ZAP1436 | Sakai: stx-, fnrS- | pDW-fSft then pDW-fSf2 |
| ZAP1437 | Sakai: stx-, mcaS- | pDW-mSft then pDW-mSf2 |
| ZAP1438 | Sakai: stx-, rprA- | pDW-rAft then pDW-rAf2 |
| ZAP1415 | Sakai:spf:tet | pDW-sfft |
| ZAP1772 | Sakai: spf (WT) | pIB-spf |
| ZAP1773 | Sakai: spf (S1) | pIB-spf-S1 |
| ZAP1774 | Sakai: spf (S2) | pIB-spf-S2 |