| pIB307 |
pMAK705-based vector for allelic exchange; temperature-sensitive replicon (61) |
| pDW-mAf2 |
pIB307 containing upstream and downstream flanking regions of micA
|
| pDW-mFf2 |
pIB307 containing upstream and downstream flanking regions of micF
|
| pDW-hBf2 |
pIB307 containing upstream and downstream flanking regions of ryhB
|
| pDW-bBf2 |
pIB307 containing upstream and downstream flanking regions of rybB |
| pDW-sff2 |
pIB307 containing upstream and downstream flanking regions of spf
|
| pDW-oAf2 |
pIB307 containing upstream and downstream flanking regions of omrA
|
| pDW-oBf2 |
pIB307 containing upstream and downstream flanking regions of omrB
|
| pDW-rAf2 |
pIB307 containing upstream and downstream flanking regions of rprA
|
| pDW-fSf2 |
pIB307 containing upstream and downstream flanking regions of fnrS
|
| pDW-mSf2 |
pIB307 containing upstream and downstream flanking regions of mcaS
|
| pDW-mAft |
pDW-mAf2 + tet cassette from pTOF61 |
| pDW-mFft |
pDW-mFf2 + tet cassette from pTOF61 |
| pDW-hBft |
pDW-hBf2 + tet cassette from pTOF61 |
| pDW-bBft |
pDW-bBf2 + tet cassette from pTOF61 |
| pDW-sfft |
pDW-sff2 + tet cassette from pTOF61 |
| pDW-oAft |
pDW-oAf2 + tet cassette from pTOF61 |
| pDW-oBft |
pDW-oBf2 + tet cassette from pTOF61 |
| pDW-rAft |
pDW-rAf2 + tet cassette from pTOF61 |
| pDW-fSft |
pDW-fSf2 + sacB/kan cassette from pDG028 |
| pDW-mSft |
pDW-mSf2 + tet cassette from pTOF61 |
| pTOF61 |
A plasmid derived from pTOF1 (62) has TcR cloned into the SmaI site |
| pDG028 |
Low-copy-number vector containing sacB/kan cassette (25) |
| pDW6 |
pAJR70 digested with BamHI/KpnI; whole sepL gene with its own promoter amplified from ZAP193 and inserted (10) |
| pDW-LEE1 |
Encodes full length Ler fusion to GFP expressed from WT promoter (33) |
| pDW-LEE5 |
Encodes full length Tir fusion to GFP expressed from WT promoter (33) |
| pDW26 |
First 51bp of sepL cloned in frame to egfp in pAJR70 (10) |
| pDW6-S1 |
Mutation of sepL 5′UTR with S1 primers |
| pDW6-S2 |
Mutation of sepL 5′UTR with S2 primers |
| pDW6-S3 |
Mutation of sepL 5′UTR with S3 primers |
| pDW6-C1 |
Mutation of sepL 5′UTR with C1 primers |
| pDW6-C2 |
Mutation of sepL 5′UTR with C2 primers |
| pDW6-C3 |
Mutation of sepL 5′UTR with C3 primers |
| pDW6-C4 |
Mutation of sepL 5′UTR with C4 primers |
| pDW6-C5 |
Mutation of sepL 5′UTR with C5 primers |
| pDW6-H1 |
Mutation of sepL 5′UTR with H1 primers |
| pIB-spf |
pIB307 containing spf plus upstream and downstream flanking regions |
| pIB-spf-S1 |
pIB-spf mutated with S1 primers |
| pIB-spf-S2 |
pIB-spf mutated with S2 primers |
