Figure 2.

The Sm site is necessary for Cajal body targeting of U1, U4 and U5 snRNAs. (A–F) WT or ΔSm U1, U4 and U5 snRNAs were transcribed in vitro and microinjected into the cytoplasm or the nucleus of HeLa cells (green). CBs are marked by coilin immunolabeling (red). Legend is the same as in Figure 1. Small red boxes in snRNA schemes represent the canonical Sm site, the orange box depicts the non-canonical U1 Sm site. (G, H) U2ΔSLI+Sm and U2 snRNA with U1-like Sm site were injected into the nucleus and the cytoplasm of Hela cells. Legend is the same as in Figure 1.