Skip to main content
. 2018 Feb 5;46(7):3774–3790. doi: 10.1093/nar/gky070

Figure 5.

Figure 5.

SmB/B′ but not the SMN protein is essential for Cajal body targeting of snRNA. (A) Depletion of the SmB/B′ protein disrupts targeting of microinjected snRNAs into the Cajal body. SmB/B′ protein was depleted by RNAi and Alexa-488 labeled WT U2 snRNA (green) subsequently microinjected into HeLa cells. Dextran-TRITC 70 kDa was used as a marker of microinjection (yellow). DNA was stained by DAPI (blue). (B, C) SMN protein was depleted by siRNA and either Alexa-488 labeled WT U2 snRNA (B) or core U2 snRNP (C) were microinjected into HeLa cells. Depletion of the SMN protein disrupts CBs, which can be rescued by cytoplasmic microinjection of core U2 snRNP (red). Coilin was used as a marker of CB (green). (C) Dotted lines mark the nucleus of microinjected cells; dashed lines mark the nucleus of non-microinjected cells. The scale bar represents 10 μm.