Figure 6.

C-terminal tails of SmB, D1 and D3 are important for Cajal body localization. Cells were transfected with plasmids expressing SmB, D1 or D3 protein variants tagged with GFP. Coilin was used as a marker of CBs (red). DNA was stained with DAPI. The scale bar represents 10 μm. Intensity of GFP signal in CBs vs. the nucleoplasm was determined by high-content microscopy. Values normalized to the WT proteins are shown in the table next to the micrographs and non-normalized values are shown in graphs. The average of three experiments and SEM are shown. Statistical significance was assayed by the two tailed t-test and data with a P value < 0.1 are marked by * and P < 0.001 by ***.