Figure 3.

Summary of the direct RNA sequencing data. (A) The histogram plot shows the distribution of read length of high quality reads obtained from yeast cell growth ethanol (magenta) and glucose (cyan), respectively, with the distribution of expected transcript lengths derived from the ORFs annotation. (B) Bar plots of the detected highly expressed transcripts are presented as an average normalized count with standard error over four biological replicates for each growth condition. The constitutively expressed, highly expressed in ethanol growth and highly expressed in glucose growth are illustrated in the left middle and right box, respectively. (C) The bubble scatter plots show the relationship between the fraction of detected full-length transcripts by the direct RNA sequencing with the transcript length and the level transcript expression. The violin-boxplots on the right show the overall distribution of the fraction of detected full-length transcripts.