Table 2. Aminoacylation kinetic parameters of mThrRS and mThrRS-L-NT for mouse tRNAThr(AGU)a.
Enzyme | K M (μM) | kcat (s−1) | k cat/KM (mM−1 s−1) |
---|---|---|---|
mThrRS | 0.74 ± 0.05 | 0.29 ± 0.04 | 391.89 |
mThrRS-L-NT | 1.31 ± 0.07 | 0.37 ± 0.05 | 282.44 |
aResults are the average of three independent repeats, with standard deviations indicated.