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. 2018 Mar 7;46(7):3612–3624. doi: 10.1093/nar/gky150

Figure 4.

Figure 4.

NH4+ enhances ASO-directed gene silencing in transfection-resistant T cell lines. (A) Treatment with NH4+ increased the efficacy of a BCL2-ASO in transfection-resistant Jurkat T cells. Cells were transfected with [50] or [100 nM] BCL2-ASO complexed with Lipofectamine® 3000 (lane 2 and 3), or treated with [1] or [2 μM] BCL2-ASO (as indicated) with or without [5 mM] NH4+ (lane 4–7) for 2 days, prior to being harvested for Western blots. α-Tubulin was used as control. (B) Treatment with NH4+ increased BCL2-ASO efficacy in CEM T cells. Cells were treated with the indicated concentrations of the BCL2-ASO with or without [5 mM] NH4+ for 2 days, prior to Western blotting. α-Tubulin was used as control.