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. 2018 Feb 22;46(7):3429–3445. doi: 10.1093/nar/gky126

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Effect of N⁴-cytosine DNA methylation on H. pylori natural transformation. Streptomycin resistant genomic DNA (200 ng) or 1487 bp long linear donor DNA (harboring kanamycin resistance cassette) was used as a donor DNA to transform the H. pylori 26695 (wild-type), R.hpyAII (ΔR), R.hpyAII-M2.hpyAII (ΔR-M2) and R.hpyAII-M1.hpyAII-M2.hpyAII (ΔR-M1-M2) deletion strains. Recombination frequency was expressed as, number of streptomycin or kanamycin resistant colonies per recipient colony-forming unit. (A) Donor DNA carrying point-mutation in rpsL gene (A128G), (B) 1487 bp linear DNA fragment carrying rdxA gene disrupted with kanamycin resistance cassette. Data was represented as mean ± SD values of pooled experiments. P-values were calculated using the Mann–Whitney U test.