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. 2018 Feb 22;46(7):3429–3445. doi: 10.1093/nar/gky126

Figure 4.

Figure 4.

Effect of N4-cytosine DNA methylation on H. pylori-induced IL-8 production and apoptosis in AGS cells. (A) H. pylori induced induction of IL-8 cytokine, AGS cells (5 × 105) were cultured in the absence (control) or presence of H. pylori (5 × 107 CFU) 26695 and the respective deletion strains, at MOI of 100 in RPMI 1640 medium containing 2% FBS, 2 mM l-glutamine. Supernatants were collected after 8 h for the quantification of IL-8 by sandwich ELISA. Experiments were performed in duplicates in two independent infections (*P <0.05). (B) H. pylori-induced apoptosis was assayed by coculturing AGS cells (3 × 106) in the absence (control) or presence of H. pylori (3 × 108 CFU) 26695 strain and its respective deletion strains for 24 h (MOI 100) in RPMI 1640 medium containing 2% FBS, 2 mM l-glutamine. Flow cytometry analysis was performed using propidium iodide staining. Apoptosis was analysed by plotting Sub-G0 population versus the strains used for coculture. Experiments were done in duplicates in three independent infections. Percentage apoptotic cells were expressed as mean ± SD. *P <0.05 as compared between groups. P values were calculated using unpaired t test.