Fig 3. ROCK inhibitor suppresses hemidesmosome formation.

(A) Ventral images of endogenous β4, their binary images, and bright field images of Y-27632-treated or untreated MCF10A cells. Cells were cultured as shown in Fig 2 and treated with 10 μM of Y-27632 or left untreated for 24 h. The red dotted lines indicate the total adhesion area defined by the bright field images. Scale bar, 20 μm. (B) Quantitative analysis of the effect of Y-27632 on HD formation. The ratio of HD area to total adhesion area was calculated, as in Fig 2. Data represent the means ± SD of 4 independent experiments (at least 7 images per experiment). **P < 0.01 (two-tailed paired t-test).