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Optimization of the of anti-human NPNT (Atlas Antibodies/Sigma) antibody using normal human kidney and breast cancer tissues. The tissues were stained at 1:50, 1:100, and 1:200 dilution, and the results show that the optimal dilution is 1:100 for the human tissues. Rabbit IgG isotype control and control without antibody (-ab) shown in lower panels. Scale bar: 50μm.
