Figure S4.

NPNT is expressed by mammary epithelial cells in MMTV-PyMT transgenic animals. (A) Quantitative real time PCR (qRT-PCR) analysis of NPNT expression levels in primary cells isolated from a normal mammary gland (FVB mouse) or from mammary tumor tissue from three different MMTV-PyMT transgenic animals (#1177, #1178, and #1179). Data are presented as mean ±SD of triplicate measurements. (B) ISH (left panel) and IHC (right panel) of serial sectioned mouse normal mammary gland (MG) and MMTV-PyMT tumor tissue at both noninvasive and invasive stages. The second and fourth rows show different images of noninvasive tumors, with higher magnification of the images in the fourth row. The sections for IHC were stained using the anti-NPNT antibody (Atlas Antibodies/Sigma). “-ab” reflects a control with no primary antibody.