Figure S6.

NPNT increases attachment in a dose-dependent manner. (A) qRT-PCR analysis of NPNT levels in mouse cell lines. Data are presented as mean ±SD of triplicate measurements from a representative experiment. (B) IHC staining of 67NR and 4T1 primary orthotopic tumors using anti-NPNT antibodies (Abnova). Staining “hot-spots” are shown. -ab reflects a control with no primary antibody. The images are representative of three animals per cell line. (C) Quantitative real-time PCR verification of NPNT knockdown in 4T1-shNPNT cells compared to 4T1-shFF control cells. Data are presented as mean ±SD of three biological replicates. * P<.05. (D) xCELLigence attachment assay of 4T1-shFF control cells and 4T1-shNPNT in xCELLigence E-plates plates coated with recombinant mouse NPNT at 0.4 μg/ml, 2 μg/ml, or 10 μg/ml prior to seeding. Data are presented as mean cell index ± SD (n=4) from a representative experiment 1 hour after seeding.