Figure 1. Schematic diagram of the vectors used in genetic engineering of mice and identification of positive transgenic animals.

(A) Schematic structures of the vectors containing the three genes (CHOP, hIAPP-CHOP and 11βHSD1-hIAPP-CHOP) used to create transgenic mice. (B) Genomic PCR positive amplification of the CHOP transgene. +, positive; −, negative; W, ddH₂O; V, positive plasmid vector; M, 100-bp DNA marker. (C) Genomic PCR positive amplification of animals carrying the CHOP (F2) and hIAPP (F3) transgenes. Mice were regarded as positive (+) when the two bands (F2 and F3) were present at the same time; otherwise, they were considered negative (−). (D) Genomic PCR positive amplification of 11β-HSD1 (F1′), CHOP(F2′) and hIAPP(F3′). If the three bands F1′, F2′  and F3′  of the object were present at the same time, the animal was considered positive. V, positive plasmid vector; W, ddH₂O; M, 100-bp DNA marker.