. Author manuscript; available in PMC: 2018 Apr 20.

Published in final edited form as: J Microbiol Methods. 2017 Feb 3;135:69–76. doi: 10.1016/j.mimet.2017.01.016

Table 1.

Raw and trimmed sequence reads following NextGen sequencing of the V4 segment of the 16S rRNA gene. The number of OTUs and calculated alpha diversity of four gut microbiome samples of D. rerio fed with control formulated diet (CFD, n = 2) and gluten formulated diet (GFD, n = 2) are listed.

	CFD1	CFD2	GFD1	GFD2	Total
Number of raw sequences	146,253	153,866	138,447	125,313	563,879
Number of sequences after trimming and filtering processes	114,804	114,916	79,906	73,594	383,220
Number of OTUs	471	439	331	322	1563
Shannon diversity	5.880	6.252	5.382	5.496	–