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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: J Immunol. 2018 Jan 31;200(6):2165–2173. doi: 10.4049/jimmunol.1701291

Figure 3. T cell-intrinsic requirement of PLDs in T cell expansion.

Figure 3

(A) A schematic diagram of the adoptive transfer procedure. A mixture of 1×105 WT OT-I cells (CD45.1+) and 1×105 PLDKO OT-I cells (CD45.2+) were mixed and transferred intravenously into WT (CD45.1+CD45.2+) recipient mice. On the following day, these recipients were infected i.v. with Lm-OVA. (B) Left panel, FACS analysis of donor naïve OT-1 T cell mixture before adoptive transfer. Right panel, FACS analysis of OT-1 T cells at day 7 after adoptive transfer. Cells were gated on live CD8+Vα2+ T cells from the spleen. (C) The percentages of WT and PLD-deficient OT-I cells among total CD8+Vα2+ T cells in the spleen after Lm-OVA infection. Data are shown as mean ± SEM. Data are representative of two independent experiments with five mice in each experiment.