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. 2018 Apr;59(5):2069–2079. doi: 10.1167/iovs.17-23758

Figure 3.

Figure 3

Analysis of particle delivery toward the back of the eye by hydrogel pushing as a function of incubation temperature and time. The optimized formulation of 1% HA in the particle formulation and 4% HA in the hydrogel formulation was injected into the SCS of ex vivo rabbit eyes. (a) Distributions of red-fluorescent particles in the SCS at (i) 0 hours, (ii) 6 hours at 4°C, (iii) 6 hours at RT, and (iv) 6 hours at 37°C after SCS injection. (b) Representative bright field (left) and fluorescence images (right) of red-fluorescent particles in the SCS at (i) 0 hours, (ii) 6 hours at 4°C, (iii) 6 hours at RT, and (iv) 6 hours at 37°C after SCS injection. The inner and the outer white dashed lines indicate the boundary of the limbus and the optic nerve, respectively. ‡APD (mm). R is reference value. ▴ indicates significant difference (1-way ANOVA, P < 0.05) between the APD of the reference (R) and the APD of the formulations. *, **Significant difference (2-way ANOVA, P < 0.05) between the 9-mm distribution of the reference (R) and other formulations. Graphs (a) present mean ± SD based on three replicate samples.