Figure 5.

Expression of PTOX, PDS, and ZDS genes during pepper (top) and tomato (bottom) fruit development. mRNA levels were determined by RT-PCR amplification of total cellular RNA. Equal amounts of total RNA were used in each reaction. The PCR products were separated by 1.5% (v/v) agarose gel electrophoresis and visualized by ethidium bromide staining. Amplification of globin mRNA (added to the RT reaction mix) was used as a control for the RT-PCR reaction (see also “Materials and Methods”). IG, Immature green; eMG, early mature green (adult size); MG, mature green; Br, breaker; IR, intermediate red (4 d after breaker); RR, red ripe (14 d after breaker).