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. 2018 Apr 20;13(4):e0192709. doi: 10.1371/journal.pone.0192709

Fig 3. RSV infection induces OPN expression in a dose dependent manner.

Fig 3

HEp-2 cells were mock-infected or infected with RSV-L19F (1 MOI). (A) Images of Hep-2 cells stained with polyclonal antibody against RSV and OPN at 48 hpi. Representative images of RSV positive (red) cells, OPN positive (green) cells, and DAPI (blue) nuclear staining at 200X magnification. (B) RSV titer by plaque assay of supernatants for increasing doses (0.1, 1 and 10 MOI) of UV-inactivated and native RSV-L19F at 48 hpi. (C) OPN protein (55 kDa) expression in HEp-2 cells at 48 hpi after mock, RSV and UV-inactivated RSV treatment in the indicated doses analyzed by western blots. β-actin (loading control) at 42 kDa was probed as the loading control.