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. 2018 Apr 20;13(4):e0192709. doi: 10.1371/journal.pone.0192709

Fig 7. OPN is a marker of high RSV loads in HEp-2 cells.

Fig 7

HEp-2 cells were mock-infected or infected with 1 MOI of RSV-L19F or rgRSV-A2. RNA, supernatants and protein were isolated 24 and 48 hpi. (A) Plaque titers were obtained from supernatants of HEp-2 cells infected with RSV-L19F or rgRSV-A2. (B—D) Expression of RSV-N, IL-1β and IFN-β were determined by qPCR. Results are presented as fold-change in expression of RSV-N, IL-1β or IFN-β mRNA normalized to the control (HPRT). (E) Western blot analysis of OPN expression. 25μg of protein lysate was loaded in each lane. OPN is seen at 55 kDa and β-actin (loading control) at 42 kDa. Experiments were performed in triplicate. * p < 0.05, ** p < 0.01, **** p < 0.0001.