Fig 2. In vitro function and localization of BM-MDSCs in vivo.

A) Representative histogram of CFSE-labeled CD8 OT-I T cells co-cultured with splenocytes and BM-MDSCs at various ratios as indicated (n = 3 in triplicate). B) RT-PCR with fold change gene expression in G-MDSCs isolated from the tumor, spleen and lungs of PyMT-WT tumor-bearing mice normalized to gene expression in BM-MDSCs (indicated by broken line; n = 3 in triplicate). C) Percent viability of BM-MDSCs at 3, 24 and 48 hours after initial DiD-labeling for 2, 5 or 10 min relative to unlabeled cells (broken line). Data represented as mean ± SEM. *p<0.05; **p<0.01; ***p<0.001. D, E) OI in vivo (D) and of ex vivo organs (E) 7 days after i.v., i.c. or i.p. injection of 1x10⁶ DiD-labeled BM-MDSCs into naïve mice. BM = bone marrow, Li = liver, LN = lymph nodes, OM = omentum majus, S = spleen, T = thymus). RE = Radiant Efficiency; R = Radiance.