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. 2018 Apr 20;13(4):e0196040. doi: 10.1371/journal.pone.0196040

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© 2018 Sceneay et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — A) Representative flow cytometry plots of the CD45.2/DiD-Cy5⁺ gated population in PyMT-WT tumors from control mice (primary tumor alone; left) and after adoptive transfer of DiD-BM-MDSCs (right) on day 24 as in Fig 5B and 5C) Flow cytometry analysis of CD11b/Ly6C/Ly6G (B) and CD11b/Ly6C subpopulations (C) as a percentage of CD45/Cy5⁺ cells within the tumor. D) Flow cytometry analysis of mature myeloid populations including granulocytes (CD11b/Ly6G), macrophages and DCs as a percentage of CD45/Cy5⁺ cells within the tumor. E) Representative flow cytometry plots of the CD45.2/DiD-Cy5⁺ gated population within the spleen of PyMT-WT tumor-bearing mice (control; left) and after adoptive transfer of DiD-BM-MDSCs (right). F,G) Flow cytometry analysis of CD11b/Ly6C/Ly6G (F) and CD11b/Ly6C subpopulations (G) as a percentage of CD45/Cy5⁺ cells within the spleen. H) Flow cytometry analysis of mature myeloid populations including granulocytes, macrophages and DCs as a percentage of CD45/Cy5⁺ cells within the spleen. Data represented as mean ± SEM; n = 6 for all groups.