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. 2018 Jan 15;29(2):123–136. doi: 10.1091/mbc.E17-04-0251

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© 2018 Samarasekera and Auld. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Csk-mediated regulation of Gliotactin is Src independent. Third-instar wing imaginal disks with ap>Gli-WT overexpression in Src -/+ mutants immunolabeled for Gliotactin (Gli, green), Dlg (red), and DAPI (blue). Dashed lines mark the boundary between dorsal and ventral compartments with the apterous side on the left. Each panel represents a single Z slice. Scale bars: 30 µm. n = 10 disks except n = 18 in D. (A–D) Overexpression of Gliotactin in (A) a Src64B[D404N]/+ heterozygous mutant background (ap>Gli-WT, Src64B[D404N]), (B) a Src64B[KO]/+ heterozygous background (ap>Gli-WT, Src64B[KO]/+), (C) a Src42A[myri]/+ heterozygous background (ap>Gli-WT, Src42A[myri]/+), and (D) a Src42A, Src64B heterozygous background (ap>Gli-WT, Src42A[myri]/+; Src64B[KO]/+). Arrows mark the leading edge of the migrating Gli overexpressing cells into the wild-type (ventral) side. Gliotactin overexpression still generated extra folds and overproliferation (stars). (E) Statistical analysis of cell migration ratios in Gliotactin overexpression alone (ap>Gli-WT) compared with expression in Src heterozygous mutant backgrounds. No significant (NS) differences were observed. Bars represent the SD (n = 15 disks).