FIGURE 8:

JAK-STAT signaling is required for BRG1 recruitment to STAT3 binding sites (SBS) at Gfap and Osmr loci. (A) ChIP- and reChIP-qPCR for STAT3 and BRG1 at the SBS of Gfap in NPCs and cells stimulated with LIF for different periods of time (LIF-stim). Each graph represents the means (±SEM) of at least three experiments. (B) ChIP-qPCR for BRG1 on the SBS of Gfap, Osmr, and Gapdh (negative control) in cells stimulated with LIF for 48 h (LIF-stim 48 h). Each graph represents the means (±SEM) of at least three experiments. (C) Model depicting BRG1 as a mediator of gene clustering of Gfap and Osmr. BRG1 and STAT3 are recruited to the SBS of Gfap and Osmr within 48 h after LIF stimulation concurrent with BRG1- and STAT3-dependent Gfap and Osmr gene clustering. Transcription of both genes is enhanced after gene clustering (at 72–96 h after LIF stimulation).