FIGURE 2:

Nuclear blebs are enriched in euchromatin and reside primarily at the major axis poles of the nucleus. (A) Fluorescence intensity ratio of nuclear bleb to nuclear body for Hoechst, H3K9ac, and H3K9ac divided by Hoechst (VPA n = 24, DZNep n = 10, and LB1–/– n = 22). Representative images are shown to the right. (B) Schematic of major vs. minor axis bleb location on the elliptical nucleus (average aspect ratio is 1.4). Average percentages of blebs that reside proximal to the major axis, within 45°. (C) Representative images of blebs on (left) and off (right) the major axis of the nucleus. Histogram of bleb location on the nuclear body relative to the major axis, denoted as angle 0, for bins of 15° (VPA n = 59, DZNep n = 50, and LB1–/– n = 63, two independent experiments each). Cells were treated with VPA or DZNep for 16–24 h. Scale bar = 10 μm. Error bars represent standard error. Asterisk denotes statistically significant difference (p < 0.01, t test).