FIGURE 5:
(A) Three normal and three scleroderma lung fibroblasts were serum starved overnight and Western blotting used to determine STAT3 phosphorylation. The ratio of pSTAT3 to STAT3 was determined by densitometry (arbitrary units). (B) Levels of STAT3 phosphorylation from A averaged for scleroderma and normal fibroblasts. Statistical analysis was done with the Student’s t test. (C) Immunohistochemistry for STAT3 in lung sections from normal volunteers and scleroderma patients.