FIGURE 7:

Scratch wound assay with a scleroderma lung fibroblast line and SD1029. The cells were plated in 24-well plates, allowed to reach confluence, and serum starved overnight with 0.1% BSA in DMEM. A scratch was made with a pipette tip and mitomycin C was added to prevent proliferation. The cells were allowed to migrate overnight with or without 10 µM SD1029. The experiment was carried out in triplicate. The area of the gap was measured with ImageJ and the percentage of closure was calculated as (area at 24 h)/(area at 0 h) × 100. The differences between untreated and treated SD1029 were not statistically significant with a Student’s t test.