Table 1. Three-state kinetic model.
Kinesin-1 and Eg5 values were added for comparison. n/m: not measured.
Construct | Unloaded Velocity | Run Length | Stall Force | Three-state model | |||||
---|---|---|---|---|---|---|---|---|---|
| |||||||||
k1 | k−1 | k20 | k3 | δ2 | vmax | ||||
| |||||||||
(nm s−1) | (nm) | (pN) | (μM−s)−1 | (s−1) | (s−1) | (s−1) | (nm) | (nm s−1) | |
Kinesin-1[34] | 671 ± 21 | 1370 ± 287 | 4.92 ± 0.08 | 2 | 120 | 12,900 | 76.86 | 5.50 | 630 |
Eg5[23,34] | 96 ± 2 | 67 ± 7 | 1.5 – 7* | 0.89 | 10 | 86 | 13.5 | 1.9 | 94 |
N700 | 76 ± 41 | 5120 ± 2900 | 3.0 ± 0.6 | 2 | 120 | 72.2 | 15.1 | 5.4 | 101 |
Kif15 | n/m | n/m | 2.7 ± 1.0 | 2 | 120 | 88.1 | 16.2 | 4.1 | 111 |
Range of values reported.