Table 1.

Differentially Expressed Genes Related to Mitochondrial Function.

Gene	EnsemblID	Leflunomide vs DMSO		A77 1726 vs DMSO		Leflunomide vs A77 1726
		Fold Changea	Adjusted P	Fold Changeb	Adjusted P	Fold Changec	Adjusted P
ATP5G1	ENSG00000159199	−1.62	0.021	1.07	0.57	−1.74	0.009
ABAT	ENSG00000183044	−1.62	0.005	−1.19	0.08	−1.37	0.012
ABCB6	ENSG00000115657	−1.59	0.048	1.21	0.30	−1.92	0.016
ABCD3	ENSG00000117528	−1.72	0.002	−1.08	0.49	−1.59	0.033
ACSS1	ENSG00000154930	−1.61	0.014	1.10	0.36	−1.76	0.007
ADPRHL2	ENSG00000116863	−1.82	0.038	−1.06	0.62	−1.72	0.020
ALDH5A1	ENSG00000112294	−1.65	0.025	−1.23	0.52	−1.34	0.267
BCL2L1	ENSG00000171552	1.30	0.075	−1.80	0.03	2.33	0.018
BIK	ENSG00000100290	19.62	0.006	−1.09	0.96	21.42	0.101
BMF	ENSG00000104081	2.62	0.011	−1.02	0.63	2.68	0.012
BNIP3	ENSG00000176171	−1.09	0.349	1.53	0.03	−1.66	0.010
CHDH	ENSG00000016391	−1.56	0.019	−1.83	0.04	1.17	0.067
CLIC1	ENSG00000213719	1.59	0.031	−1.13	0.18	1.81	0.010
CNP	ENSG00000173786	−1.74	0.018	−1.05	0.73	−1.66	0.034
COQ10B	ENSG00000115520	2.48	0.004	1.41	0.10	1.75	0.025
COQ4	ENSG00000167113	−1.56	0.044	1.04	0.69	−1.63	0.025
CPT1A	ENSG00000110090	4.30	0.017	1.50	0.09	2.88	0.015
CPT2	ENSG00000157184	−1.53	0.011	−1.39	0.06	−1.10	0.255
CYP24A	ENSG00000019186	7.70	0.015	−1.86	0.04	14.31	0.005
DDX28	ENSG00000182810	−1.04	0.641	−1.86	0.04	1.79	0.015
DHODH	ENSG00000102967	−1.02	0.887	−1.65	0.05	1.63	0.012
DHRS1	ENSG00000157379	−1.79	0.016	1.56	0.06	−2.79	0.005
DNAJC19	ENSG00000205981	−1.65	0.011	1.00	0.98	−1.65	0.006
DYNLL1	ENSG00000088986	−1.83	0.010	−1.01	0.74	−1.81	0.005
DYNLL2	ENSG00000264364	−1.90	0.045	−1.26	0.28	−1.51	0.098
FARS2	ENSG00000145982	1.09	0.425	−1.91	0.03	2.08	0.007
GRPEL2	ENSG00000164284	2.33	0.006	1.05	0.60	2.23	0.006
HK2	ENSG00000159399	3.24	0.009	−1.04	0.65	3.36	0.004
HRK	ENSG00000135116	2.08	0.046	−1.95	0.72	4.05	0.509
HSPA1L	ENSG00000204390	−2.37	0.207	−2.12	0.05	−1.12	0.798
HSPE1	ENSG00000115541	−1.59	0.023	−1.03	0.65	−1.55	0.009
LGALS3	ENSG00000131981	2.28	0.019	1.14	0.34	2.00	0.034
MALSU1	ENSG00000156928	−1.58	0.043	−1.06	0.52	−1.49	0.030
MCL1	ENSG00000143384	2.84	0.003	−1.13	0.20	3.21	0.001
MGEA5	ENSG00000198408	2.58	0.044	1.26	0.09	2.06	0.038
MIEF2	ENSG00000177427	−2.33	0.048	−1.15	0.54	−2.03	0.083
MLYCD	ENSG00000103150	−2.05	0.008	−1.44	0.04	−1.42	0.019
MMAB	ENSG00000139428	−1.59	0.038	−1.11	0.54	−1.44	0.044
MRM2	ENSG00000122687	−2.00	0.024	−1.37	0.17	−1.46	0.047
MRPL49	ENSG00000149792	−1.64	0.027	1.11	0.21	−1.81	0.016
MRPL51	ENSG00000111639	−1.71	0.031	1.01	0.91	−1.72	0.002
MRPS17	ENSG00000239789	−1.65	0.027	−1.23	0.13	−1.35	0.007
MRPS18C	ENSG00000163319	−1.52	0.037	−1.12	0.46	−1.36	0.034
MRPS21	ENSG00000187145	−1.56	0.026	−1.13	0.37	−1.38	0.037
MRPS26	ENSG00000125901	−1.51	0.014	−1.01	0.87	−1.50	0.013
MRPS30	ENSG00000112996	1.59	0.028	−1.12	0.31	1.78	0.035
MRPS31	ENSG00000102738	−1.53	0.044	−1.07	0.37	−1.44	0.030
MTERF1	ENSG00000127989	−2.55	0.036	1.19	0.42	−3.02	0.008
MTERF2	ENSG00000120832	−4.17	0.006	−1.02	0.86	−4.07	0.004
MTFR2	ENSG00000146410	−1.02	0.877	−2.15	0.04	2.12	0.023
MTX3	ENSG00000177034	1.55	0.027	−1.07	0.46	1.66	0.028
NDUFAF5	ENSG00000101247	1.67	0.012	−1.33	0.16	2.22	0.028
OAT	ENSG00000065154	1.17	0.026	1.63	0.03	−1.40	0.024
PDP2	ENSG00000172840	−1.66	0.018	1.34	0.10	−2.23	0.013
PIM2	ENSG00000102096	−2.92	0.034	1.56	0.09	−4.55	0.005
PLD6	ENSG00000179598	1.65	0.026	−1.05	0.68	1.74	0.015
PMAIP1	ENSG00000141682	2.10	0.045	1.94	0.19	1.08	0.794
POLG	ENSG00000140521	−1.57	0.013	1.04	0.65	−1.62	0.020
PPARGC1A	ENSG00000109819	2.11	0.049	1.04	0.76	2.02	0.032
PPP3CC	ENSG00000120910	1.82	0.048	−1.10	0.81	2.01	0.191
RAB11FIP5	ENSG00000135631	−1.62	0.016	1.22	0.11	−1.97	0.014
RAD51	ENSG00000051180	−1.52	0.018	1.01	0.79	−1.53	0.024
RNF144B	ENSG00000137393	1.73	0.017	−2.48	0.11	4.30	0.038
SDHAF1	ENSG00000205138	−1.99	0.047	1.04	0.86	−2.08	0.023
SDHAF2	ENSG00000167985	−1.51	0.027	−1.09	0.54	−1.40	0.041
SFXN2	ENSG00000156398	−1.84	0.016	1.16	0.31	−2.12	0.007
SFXN3	ENSG00000107819	−1.79	0.010	−1.06	0.65	−1.69	0.016
SFXN5	ENSG00000144040	−1.78	0.010	1.20	0.07	−2.13	0.004
SH3GLB1	ENSG00000097033	1.96	0.026	1.06	0.35	1.85	0.022
SLC25A14	ENSG00000102078	−1.84	0.012	1.01	0.81	−1.86	0.016
SLC25A19	ENSG00000125454	−1.92	0.012	−1.25	0.13	−1.54	0.051
SLC25A25	ENSG00000148339	9.50	0.003	1.80	0.04	5.29	0.001
SLC25A29	ENSG00000197119	−2.85	0.026	1.13	0.38	−3.22	0.017
SLC25A33	ENSG00000171612	1.98	0.006	1.35	0.13	1.47	0.031
SLC25A36	ENSG00000114120	1.88	0.021	1.07	0.65	1.76	0.056
SNN	ENSG00000184602	−2.89	0.006	1.39	0.06	−4.01	0.004
STARD1	ENSG00000133121	3.63	0.008	−1.62	0.06	5.90	0.003
TFB1M	ENSG00000029639	−1.13	0.444	−1.65	0.02	1.46	0.098
TFB2M	ENSG00000162851	−1.66	0.006	−1.25	0.05	−1.33	0.015
TIMM10B	ENSG00000132286	−1.99	0.005	1.30	0.06	−2.59	0.006
TIMM21	ENSG00000075336	−2.04	0.001	−1.17	0.22	−1.74	0.026
TIMM44	ENSG00000104980	−1.51	0.048	−1.02	0.73	−1.48	0.065
TIMM8A	ENSG00000126953	−1.96	0.006	−1.17	0.11	−1.67	0.008
TRIAP1	ENSG00000170855	−1.68	0.007	1.09	0.24	−1.82	0.004
TUSC2	ENSG00000114383	−2.15	0.019	1.01	0.88	−2.18	0.022

Fold change was calculated as the ratio of the mean expression levels of the gene between two groups. A positive value of fold change indicates a relatively higher gene expression level while a negative value of fold change indicates a relatively lower gene expression level in

^aHepG2 cells treated with leflunomide (125 μM) as compared to HepG2 cells treated with DMSO (vehicle control)

^bHepG2 cells treated with A77 1726 (125 μM) as compared to HepG2 cells treated with DMSO (vehicle control), or

^cHepG2 cells treated with leflunomide (125 μM) as compared to HepG2 cells treated with A77 1726 (125 μM) for 6 h. Each treatment group contains four replicates. Statistically significant changes (absolute fold change > 1.5; adjusted P < 0.05) were marked in bold.