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. Author manuscript; available in PMC: 2018 Apr 20.
Published in final edited form as: J Vet Cardiol. 2015 Dec;17(Suppl 1):S53–S73. doi: 10.1016/j.jvc.2015.03.001

Figure 5.

Figure 5

Incorporation of the A31P cMyBP-C missense mutation in sarcomeres of A31P-affected cats. Top Row, Immunofluorescent localization of cMyBP-C (green) was performed using a polyclonal antibody against cMyBPC. The antibody recognized cMyBP-C in myofibrils from unaffected wild-type (A), heterozygous (B), and homozygous A31P cats (C). Counterstaining with an antibody to myomesin (red) in A indicates M-line position. Bottom Row, Immunofluorescent localization of A31P mutant cMyBP-C (green) was performed using an affinity-purified antibody specific for the A31P mutation. The antibody did not cross-react with wild-type cMyBP-C in myofibrils from unaffected wild-type cats (D) but recognized A31P cMyBP-C in myofibrils from heterozygous (E) and homozygous (F) A31P cats. Counterstaining with an antibody to myomesin (red) indicates M-line position in D. G, Merged image of the same myofibril as shown in F showing counterstaining with an antibody to myomesin (red) to indicate M-line position. (From Harris et al. Circ Res 2011;108 (6): 751–764 with permission).