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. Author manuscript; available in PMC: 2018 Apr 20.
Published in final edited form as: Methods Mol Biol. 2017;1519:325–331. doi: 10.1007/978-1-4939-6581-6_22

Table 2.

Calculations of bacterial amounts needed to infect macrophages at desired MOI.

MOI 1:1 MOI 2:1 MOI 4:1 MOI 5:1 MOI 10:1
Amount of bacteria needed to be added to 1.5 × 107 cells in 75 cm2 flask 1.5 × 107 3 × 107 6 × 107 7.5 × 107 1.5 × 108
Original volume of bacteria at OD600 0.6 needed to be pelleted, ml 0.5 0.5 1 1 2
Total volume of BUB that needs to be added to bacterial pellet, ml 10 5 5 4 4
Concentration of bacteria in BUB suspension, bacteria/ml 5 × 106 1 × 107 2 × 107 2.5 × 107 5 × 107
Volume of bacterial suspension needed to be added to 1.5 × 107 cells in 75 cm2 flask, ml 3 3 3 3 3