Figure 3.
Male and female 4-week-old NPY-hrGFP mice were tested for ARC amylin response. A: ARC amylin-induced p-ERK 45 min after saline or amylin injection (50 μg/kg i.p.). B: Percentage of p-ERK–activated NPY neurons. C: 20× representative picture of the ARC after saline or amylin injection. p-ERK neurons are in red, and NPY-hrGFP neurons are in green. N = 8/group. Data are means ± SEM. ***P < 0.001 vs. saline-treated rats after Student t test. 3V, third ventricle; Avg/sec, average per section.