Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| strain, strain background (Mouse) |
C57BL/6 | Charles River | Cat#000664; RRID:IMSR_JAX:000664 |
|
| strain, strain background (Mouse) |
B6.SJL-Slc6a3 tm1.1(cre)Bkmn/J | Jackson Laboratory | Cat#006660; RRID: IMSR_JAX:006660 |
|
| strain, strain background (Mouse) |
B6.129S-Oxttm1.1(cre)Dolsn/J | Jackson Laboratory | Cat#024234; RRID:IMSR_JAX:024234 |
|
| strain, strain background (Mouse) |
Slc17a6 tm2(cre)Lowl/J | Jackson Laboratory | Cat#016963; RRID:IMSR_JAX:016963 |
|
| strain, strain background (Mouse) |
B6.Cg-Gt(ROSA) 26Sortm14(CAG-tdTomato)Hze/J |
Jackson Laboratory | Cat#007914; RRID:IMSR_JAX:007914 |
|
| strain, strain background (Mouse) |
B6.129S-Gt(ROSA)26Sortm32
(CAG-COP4*H134R/EYFP)Hze/J |
Jackson Laboratory | Cat#012569; RRID:IMSR_JAX:012569 |
|
| antibody | Rabbit anti-tyrosine hydroxylase |
Millipore | Cat#AB152; RRID:AB_390204 |
concentration: 1:1000 |
| antibody | Rabbit anti-oxytocin receptor (OxtR-2) |
NA | NA | Gift from R. Froemke (Marlin et al., 2015; Mitre et al., 2016) |
| recombinant DNA reagent | AAV9-EF1a-DIO-hChR2 (H134R)-mCherry |
UPenn viral core | CS0543-3CS | |
| commercial assay or kit | RNAscope Fluorescence Multiplex Assay |
ACDBio | ||
| commercial assay or kit | RNAscope Probe- Mm-Cnr1 | ACDBio | Cat#420721-C1 | |
| commercial assay or kit | RNAscope Probe- Mm-Th | ACDBio | Cat#317621-C2 | |
| commercial assay or kit | RNAscope Probe- Mm-Slc17a6 | ACDBio | Cat#319171-C2 | |
| commercial assay or kit | RNAscope Probe- Mm-Slc17a7 | ACDBio | Cat#416631-C2 | |
| commercial assay or kit | RNAscope Probe- Mm-Oxtr | ACDBio | Cat#412171-C1 | |
| chemical compound, drug | Oxytocin | Tocris | Cat#1910; CAS 50-56-6 | |
| chemical compound, drug | L-368,899 hydrochloride | Tocris | Cat#2641; CAS 160312-62-9 | |
| chemical compound, drug | SR 49059 | Tocris | Cat#2310; CAS 150375-75-0 | |
| chemical compound, drug | NBQX disodium salt | Tocris | Cat#1044; CAS 479347-86-9 | |
| chemical compound, drug | (RS)-CPP | Tocris | Cat#0173; CAS 100828-16-8 | |
| chemical compound, drug | SR95531 hydrobromide | Tocris | Cat#1262; CAS 104104-50-9 | |
| chemical compound, drug | U73122 | Tocris | Cat#1268; CAS 112648-68-7 | |
| chemical compound, drug | CP945598 hydrochloride | Tocris | Cat#4236; CAS 686347-12-6 | |
| chemical compound, drug | WIN55212-2 | Tocris | Cat#1038; CAS 131543-23-2 | |
| chemical compound, drug | AM251 | Tocris | Cat#1117; CAS 183232-66-8 | |
| chemical compound, drug | orlistat (THL) | Tocris | Cat#3540; CAS 96829-58-2 | |
| chemical compound, drug | MNI-L-glutamate | Tocris | Cat#1490; CAS 295325-62-1 | |
| chemical compound, drug | Alexa Fluor 488 hydrazide | Thermo Fisher Scientific | Cat#A10436 | |
| chemical compound, drug | Alexa Fluor 594 hydrazide | Thermo Fisher Scientific | Cat#A10438 | |
| chemical compound, drug | Green retrobeads (GRB) | Lumafluor Inc. | Cat#G180 | |
| software, algorithm | GraphPad Prizm 5 | GraphPad | RRID:SCR_002798 | |
| software, algorithm | FIJI | Schindelin et al., 2012 |
http://fiji.sc/; RRID:SCR_002285 |
|
| software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 | |
| software, algorithm | Igor Pro | Wavemetrics | RRID:SCR_000325 | |
| software, algorithm | SPSS | IBM | RRID:SCR_002865 |