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. 2018 Mar 16;7:e35264. doi: 10.7554/eLife.35264

Figure 10. Serotonin regulates PPL1-γ2α′1 and PPL1-γ1pedc DANs via different receptors.

The yeast food-seeking performance of 24-hr-starved (A and C–F) and food-satiated (B) male flies was assessed. (A) The performance of R50H05-GAL4;UAS-shits1 flies was statistically worse than for the control flies at a restrictive 32°C (5-HT neurons, Kruskal-Wallis, n = 30, p<0.0001). (B) The performance of R50H05-GAL4;UAS-TrpA1 flies was statistically better than for the control flies at a restrictive 32°C (5-HT neurons, Kruskal-Wallis, n = 30, p<0.0001). (C) The performance of MB296B;UAS-5HT1B-RNAi flies was statistically worse than for the control flies (PPL1-γ2α′1, Kruskal-Wallis, n = 39–40, p=0.0003). (D) The performance of MB320C;UAS-5HT1B-RNAi flies was not statistically different from that of control flies (PPL1-γ1pedc, Kruskal-Wallis, n = 30, p>0.9999). (E) The performance of MB296B;UAS-5HT2A-RNAi flies was not statistically different from that of control flies (PPL1-γ2α′1 Kruskal-Wallis, n = 30, p=0.5116). (F) The performance of MB320C;UAS-5HT2A-RNAi flies was statistically worse than that of control flies (PPL1-γ1pedc, Kruskal-Wallis, n = 30, p=0.0263). Individual data points and mean ± SEM are shown.

Figure 10.

Figure 10—figure supplement 1. Expression of UAS-shits1 or UAS-TrpA1 in serotoninergic neurons does not affect yeast food-seeking performance at the permissive temperature.

Figure 10—figure supplement 1.

(A) No statistical difference was detected between flies expressing UAS-shits1 in serotoninergic neurons and the relevant controls (R50H05, Kruskal-Wallis, n = 20, p=0.5377). (B) No statistical difference was detected between flies expressing UAS-TrpA1 in serotoninergic neurons and the relevant controls (R50H05, Kruskal-Wallis, n = 20, p=0.6367). Individual data points and mean ± SEM are shown. Satiety states (fed or hungry) are indicated in each figure.
Figure 10—figure supplement 2. RNAi knockdown of serotonin receptors in the yeast-seeking DANs.

Figure 10—figure supplement 2.

The yeast food-seeking performance of 24-hr-starved male flies with RNAi knockdown of different serotonin receptors (5HT1A, 5HT1B, 5HT2A, 5HT2B, and 5HT7) in different DANs is shown. Data are individual data points and mean ± SEM. The names of the GAL4 lines (MB296B, MB058B, MB087C, MB320C, MB301B, and MB630B) and the neurons they label (PPL1-γ2α′1, PPL1-α′2α2, PAM-β′2a, PPL1-γ1pedc, PAM-β2β′2a, and PPL1-α3 DANs) are indicated. For all combinations shown here, there is no significant difference in yeast-seeking performance between RNAi knockdown flies and at least one of their relevant controls (Kruskal-Wallis, n = 25–30, p>0.05), except for flies with knockdown of 5HT7 in PPL1-α′2α2 DANs that exhibited slightly enhanced yeast food-seeking performance (Kruskal-Wallis, n = 30, p=0.0099). The latter finding suggests that PPL1-α′2α2 DANs may be regulated by tonic inhibitory serotonin inputs.
Figure 10—figure supplement 3. A second set of RNAi lines confirms the importance of 5HT1B and 5HT2A receptors in regulating yeast food-seeking behavior.

Figure 10—figure supplement 3.

(A) The yeast food-seeking performance of MB296B;UAS-5HT1B-RNAi2 flies was statistically worse than that of the control flies (PPL1-γ2α′1, Kruskal-Wallis, n = 30, p=0.0012). (B) The yeast food-seeking performance of MB320C;UAS-5HT2A-RNAi2 flies was statistically worse than that of the control flies (PPL1-γ1pedc, Kruskal-Wallis, n = 28–30, p=0.0388). Individual data points and mean ± SEM are shown.