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. 2018 Mar 16;7:e35264. doi: 10.7554/eLife.35264

Figure 2. Kenyon cells are required for yeast food-seeking behavior in hungry flies.

(A–C) Male flies starved for 24 hr were assessed for their yeast food-seeking performance. The performance of GAL4;UAS-shits1 flies was statistically different from the controls for (A) MB005B split-GAL4 (α′β′ KCs; Kruskal-Wallis, n = 30, p<0.0001), (B) MB008B split-GAL4 (αβ KCs; Kruskal-Wallis, n = 30, p<0.0001), and (C) MB131B split-GAL4 (γ KCs; Kruskal-Wallis, n = 30, p=0.0003). Individual data points and mean ± SEM are shown. The brain images are full z-projections of confocal stacks showing the expression patterns of the GAL4 lines (green) counter-stained with nc82 antibody (magenta). Insets are z-projections of the MB lobes. Scale bars are 100 µm.

Figure 2.

Figure 2—figure supplement 1. Expression of UAS-shits1 in the KCs does not affect yeast food-seeking behavior in hungry flies at the permissive temperature.

Figure 2—figure supplement 1.

(A) No statistical difference was detected between flies expressing UAS-shits1 in α′β′ KCs and relevant controls (MB005B, Kruskal-Wallis, n = 20, p=0.7165). (B) No statistical difference was detected between flies expressing UAS-shits1 in αβ KCs and relevant controls (MB008B, Kruskal-Wallis, n = 20, p=0.7077). (C) No statistical difference was detected between flies expressing UAS-shits1 in γ KCs and relevant controls (MB131B, Kruskal-Wallis, n = 20, p=0.4803). Individual data points and mean ± SEM are shown.