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. 2018 Mar 16;7:e35264. doi: 10.7554/eLife.35264

Figure 3. Five MBONs are required for yeast food-seeking behavior in hungry flies.

Male flies starved for 24 hr were assessed for the yeast food-seeking performance. The performance of GAL4;UAS-shits1 flies was statistically lower than the controls for (A) MB112C split-GAL4 (MBON-γ1pedc>αβ, Kruskal-Wallis, n = 30, p<0.0001), (B) MB077B split-GAL4 (MBON-γ2α′1, Kruskal-Wallis, n = 30, p<0.0001), (C) MB091C split-GAL4 (MBON-α′2, Kruskal-Wallis, n = 30, p=0.0004), (D) MB399B split-GAL4 (MBON-β2β′2a, Kruskal-Wallis, n = 30, p<0.0001), and (E) G0239-GAL4 (MBON-α3, Kruskal-Wallis, n = 30, p=0.0002). Individual data points and mean ± SEM are shown. The brain images are full z-projections of confocal stacks showing the expression patterns of the GAL4 lines (green) counter-stained with nc82 antibody (magenta). One side of the MB is outlined by a white dashed line. Scale bars are 100 µm.

Figure 3.

Figure 3—figure supplement 1. Effects on yeast food-seeking behavior in hungry flies when GAL4 lines labeling different MBONs are used to drive UAS-shits1 expression.

Figure 3—figure supplement 1.

The MBONs labeled by each GAL4 line are indicated. The MBONs potentially involved in hunger-driven food seeking behavior are shown in bold font (the first six MBONs from the left). MBONs with positive or negative valences are in red or blue fonts, respectively (Aso et al., 2014b). All the GAL4;UAS-shits1 flies that showed impaired food seeking at 32°C performed normally at a permissive 23°C.
Figure 3—figure supplement 1—source data 1. Source file for the table in Figure 3—figure supplement 1.
elife-35264-fig3-figsupp1-data1.xlsx (12.7KB, xlsx)
DOI: 10.7554/eLife.35264.007
Figure 3—figure supplement 2. Blocking the MBONs required for yeast food-seeking behavior does not affect the locomotion of flies.

Figure 3—figure supplement 2.

The average speed for GAL4;UAS-shits1 flies at a restrictive 32°C is shown. No significant difference was detected (Kruskal-Wallis, 6 ≤ n ≤ 28, p>0.9999). Individual data points and mean ± SEM are shown.
Figure 3—figure supplement 3. Expression of UAS-shits1 in the five MBONs does not affect yeast food-seeking behavior in hungry flies at the permissive temperature.

Figure 3—figure supplement 3.

No statistical difference was detected between relevant controls and flies expressing UAS-shits1 in (A) MBON-γ1pedc>αβ (MB112C, Kruskal-Wallis, n = 20, p=0.8705), (B) MBON-γ2α′1 (MB077B, Kruskal-Wallis, n = 20, p=0.6004), (C) MBON-α′2 (MB091C, Kruskal-Wallis, n = 20, p=0.5172), (D) MBON-β2β′2a (MB399B, Kruskal-Wallis, n = 20, p=0.0762), and (E) MBON-α3 (G0239, Kruskal-Wallis, n = 20, p=0.7276). Individual data points and mean ± SEM are shown.
Figure 3—figure supplement 4. The five MBONs and the KCs are required during the seeking phase in our food-seeking assay.

Figure 3—figure supplement 4.

Male flies starved for 24 hr were tested for their yeast food-seeking performance. In these experiments, a fly was considered as finding the food target whenever it touched the yeast drop. The performance of GAL4;UAS-shits1 flies was significantly lower than the controls for (A) MB112C split-GAL4 (MBON-γ1pedc>αβ, Kruskal-Wallis, n = 30, p<0.0001), (B) MB077B split-GAL4 (MBON-γ2α′1, Kruskal-Wallis, n = 30, p<0.0001), (C) MB091C split-GAL4 (MBON-α′2, Kruskal-Wallis, n = 29–30, p<0.0001), (D) MB399B split-GAL4 (MBON-β2β′2a, Kruskal-Wallis, n = 30, p=0.0003), (E) G0239-GAL4 (MBON-α3, Kruskal-Wallis, n = 30, p<0.0001), (F) MB005B split-GAL4 (α′β′ KCs; Kruskal-Wallis, n = 30, p<0.0001), (G) MB008B split-GAL4 (αβ KCs; Kruskal-Wallis, n = 30, p<0.0001), and (H) MB131B split-GAL4 (γ KCs; Kruskal-Wallis, n = 30, p<0.0001). Individual data points and mean ± SEM are shown.