Skip to main content
. 2018 Mar 16;7:e35264. doi: 10.7554/eLife.35264

Key resources table.

Reagent type Designation Source or reference Identifiers Additional information
Fly line E0067-Gal4 (Pai et al., 2013) NA Gift from Ann-Shyn Chiang
Fly line G0239-Gal4 (Pai et al., 2013) Flybase: FBti0132502
Fly line MB002B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68305
Fly line MB005B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68306
Fly line MB008B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68291
Fly line MB011B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68294
Fly line MB018B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68296
Fly line MB027B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68301
Fly line MB050B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68365
Fly line MB051B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68275
Fly line MB052B-SplitGal4 (Aso et al., 2014a) NA Gift from Yoshinori Aso
Fly line MB057B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68277
Fly line MB062C-SplitGal4 (Aso et al., 2014a) NA Gift from Yoshinori Aso
Fly line MB077B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68283
Fly line MB077C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68284
Fly line MB080C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68285
Fly line MB082C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68286
Fly line MB083C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68287
Fly line MB085C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68288
Fly line MB090C-SplitGal4 (Aso et al., 2014a) NA Gift from Yoshinori Aso
Fly line MB091C-SplitGal4 (Aso et al., 2014a) NA Gift from Yoshinori Aso
Fly line MB093C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68289
Fly line MB110C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68262
Fly line MB112C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68263
Fly line MB131B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68265
Fly line MB210B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68272
Fly line MB242A-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68307
Fly line MB262B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68254
Fly line MB298B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68309
Fly line MB310C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68313
Fly line MB399B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68369
Fly line MB433B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68324
Fly line MB434B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68325
Fly line MB542B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68372
Fly line MB543B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68335
Fly line MB549C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68373
Fly line MB622B-SplitGal4 (Aso et al., 2014a) NA Gift from Yoshinori Aso
Fly line R21D02-Gal4 (Owald et al., 2015) RRID:BDSC_48939
Fly line R50H05-Gal4 (Albin et al., 2015) RRID:BDSC_38764
Fly line R66C08-Gal4 (Owald et al., 2015) RRID:BDSC_49412
Fly line VT1211-Gal4 (Owald et al., 2015) VDRC: 202324
Fly line VT999036-Gal4 (Aso et al., 2014a) NA Gift from Yoshinori Aso
Fly line MB320C-SplitGAL4 (Aso et al., 2014a) RRID:BDSC_68253
Fly line MB058B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68278
Fly line MB087C-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68366
Fly line MB296B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68308
Fly line MB301B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68311
Fly line MB630B-SplitGal4 (Aso et al., 2014a) RRID:BDSC_68334
Fly line *UAS-DAMBi (Pimentel et al., 2016) VDRC: 105324; RRID:FlyBase_FBst0478846
Fly line *UAS-GADi (Barnstedt et al., 2016) VDRC: 32344; RRID:FlyBase_FBst0459538
Fly line *UAS-Rdli (Cheung and Scott, 2017) VDRC: 41103; RRID:FlyBase_FBst0463935
Fly line UAS-5HT1Ai (Lee et al., 2011) VDRC: 106094; RRID:FlyBase_FBst0472248
Fly line *UAS-5HT1Bi (Mohammad et al., 2016) VDRC: 109929; RRID:FlyBase_FBst0476393
Fly line *UAS-5HT2Ai (Mohammad et al., 2016) VDRC: 102105; RRID:FlyBase_FBst0475582
Fly line UAS-5HT2Bi (Mohammad et al., 2016) VDRC: 102356; RRID:FlyBase_FBst0478281
Fly line UAS-5HT7i (Dietzl et al., 2007) VDRC: 104804; RRID:FlyBase_FBst0472736
Fly line *UAS-NPFRi (Ni et al., 2009) RRID:BDSC_25939
Fly line *UAS-sNPFRi (Hong et al., 2012) VDRC: 38925; RRID:FlyBase_FBst0462758
Fly line *UAS-dInRi (Loh et al., 2017) RRID:BDSC_35251
Fly line *UAS-DAR1i (Yamagata et al., 2016) NA Gift from Hiromu Tanimoto
Fly line *UAS-DAMBi2 (Hattori et al., 2017) RRID:BDSC_51423
Fly line *UAS-GADi2 (Koganezawa et al., 2016) RRID:BDSC_51794
Fly line *UAS-Rdli2 (Koganezawa et al., 2016) RRID:BDSC_52903
Fly line *UAS-5HT1Bi2 (Kaneko et al., 2017) RRID:BDSC_33418
Fly line *UAS-5HT2A2 (Ni et al., 2009) RRID:BDSC_56870
Fly line *UAS-NPFRi2 (Dietzl et al., 2007) VDRC: 107663; RRID:FlyBase_FBst0481454
Fly line *UAS-dInRi2 (Ni et al., 2009) RRID:BDSC_51518
Fly line *UAS-sNPFRi2 (Hu et al., 2017) RRID:BDSC_27507
Fly line *UAS-DAR1i2 (Yamagata et al., 2016) NA Gift from Hiromu Tanimoto
Fly line Orco2 (Larsson et al., 2004) RRID:BDSC_23130
Fly line IR25a2 (Abuin et al., 2011) RRID:BDSC_41737
Fly line IR8a1 (Abuin et al., 2011) RRID:BDSC_41744
Fly line DAMB (Selcho et al., 2009) Flybase: FBab0047678
Fly line DopR1 (dumb2) (Qin et al., 2012) Exelixis: f02676; RRID:FlyBase_FBst1017920
Fly line D2Rf06521 (Marella et al., 2012) Exelixis: f06521; RRID:FlyBase_FBst1020637
Fly line DopEcRc02142 (Inagaki et al., 2012) Exelixis: c02142; RRID:FlyBase_FBst1006135
Fly line UAS-TrpA1 (Hamada et al., 2008) Flybase: FBtp0040248 Gift from Scott Waddell
Fly line UAS-shits1 (Kitamoto, 2001) Flybase: FBtp0013545 Gift from Scott Waddell
Fly line UAS-mCD8-GFP (Lee and Luo, 1999) Flybase: FBtp0002652 Gift from Scott Waddell
Fly line UAS-GCaMP6m (Chen et al., 2013) RRID:BDSC_42748
Fly line UAS-DenMark,UAS-Dsyd-1::GFP (Owald et al., 2015) NA Gift from Scott Waddell
Fly line nSyb-GAL4 (Pauli et al., 2008) Flybase: FBtp0041245 Gift from Scott Waddell
Fly line da-GAL4 (Wang et al., 2017) RRID:BDSC_55851
Antibody Mouse anti-brp (nc82) Developmental Studys Hybridoma Bank (DSHB), IA, USA RRID:AB_2314866
Antibody Chicken anti-GFP Abcam, UK RRID:AB_300798 1:5000
Antibody Rabbit anti-Dsred Clontech, CA, USA RRID:AB_10013483 1:500
Antibody Rat anti-mCD8α Thermo Fisher Scientific, MA, USA RRID:AB_10392843 1:100
Antibody Goat anti-rabbit (Cy3) Jackson ImmunoResearch, PA, USA RRID:AB_2338006 1:400
Antibody Goat anti-mouse (Cy3) Jackson ImmunoResearch, PA, USA RRID:AB_2338692 1:400
Antibody Donkey anti-chicken (Alexa 488) Jackson ImmunoResearch, PA, USA RRID:AB_2340375 1:400
Antibody Goat anti-rat (Alexa 488) Thermo Fisher Scientific, MA, USA RRID:AB_141373 1:400
Food odor Dry yeast Ferminpan red, Italy NA
Food odor Apple cider vinegar Alce Nero, Italy NA
Food odor Banana powder Gen Asia Biotech, Taiwan NA
Chemical Sucrose Merck, Germany Cat# 107687
Chemical Agar BD, NJ, USA Cat# 214530
Chemical Formaldehyde Sigma, MO, USA Cat# F8775
Chemical PBS Sigma, MO, USA Cat# P4417
Chemical Gold Antifade reagent Thermo Fisher Scientific, MA, USA Cat# S36937
Chemical paraffin wax Sigma, MO, USA Cat# 327304
Serum Normal goat serum Jackson ImmunoResearch, PA, USA RRID:AB_2336990
Kits TRIzol RNA Isolation reagents Thermo Fisher Scientific, MA, USA Cat# 15596026
Kits SuperScript IV First-Strand Synthesis System for RT-PCR Kit Thermo Fisher Scientific, MA, USA Cat# 18091050
Software Prism 7 GraphPad, CA, USA RRID:SCR_002798
Software MATLAB 2017a MathWorks, MA, USA RRID:SCR_001622
Software Fiji/ImageJ Fiji RRID:SCR_002285

*Asterisks indicate the RNAi lines whose knockdown efficiencies are shown in Figure 6—figure supplement 3.