Key resources table.
Reagent type | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Fly line | E0067-Gal4 | (Pai et al., 2013) | NA | Gift from Ann-Shyn Chiang |
Fly line | G0239-Gal4 | (Pai et al., 2013) | Flybase: FBti0132502 | |
Fly line | MB002B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68305 | |
Fly line | MB005B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68306 | |
Fly line | MB008B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68291 | |
Fly line | MB011B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68294 | |
Fly line | MB018B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68296 | |
Fly line | MB027B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68301 | |
Fly line | MB050B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68365 | |
Fly line | MB051B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68275 | |
Fly line | MB052B-SplitGal4 | (Aso et al., 2014a) | NA | Gift from Yoshinori Aso |
Fly line | MB057B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68277 | |
Fly line | MB062C-SplitGal4 | (Aso et al., 2014a) | NA | Gift from Yoshinori Aso |
Fly line | MB077B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68283 | |
Fly line | MB077C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68284 | |
Fly line | MB080C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68285 | |
Fly line | MB082C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68286 | |
Fly line | MB083C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68287 | |
Fly line | MB085C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68288 | |
Fly line | MB090C-SplitGal4 | (Aso et al., 2014a) | NA | Gift from Yoshinori Aso |
Fly line | MB091C-SplitGal4 | (Aso et al., 2014a) | NA | Gift from Yoshinori Aso |
Fly line | MB093C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68289 | |
Fly line | MB110C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68262 | |
Fly line | MB112C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68263 | |
Fly line | MB131B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68265 | |
Fly line | MB210B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68272 | |
Fly line | MB242A-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68307 | |
Fly line | MB262B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68254 | |
Fly line | MB298B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68309 | |
Fly line | MB310C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68313 | |
Fly line | MB399B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68369 | |
Fly line | MB433B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68324 | |
Fly line | MB434B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68325 | |
Fly line | MB542B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68372 | |
Fly line | MB543B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68335 | |
Fly line | MB549C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68373 | |
Fly line | MB622B-SplitGal4 | (Aso et al., 2014a) | NA | Gift from Yoshinori Aso |
Fly line | R21D02-Gal4 | (Owald et al., 2015) | RRID:BDSC_48939 | |
Fly line | R50H05-Gal4 | (Albin et al., 2015) | RRID:BDSC_38764 | |
Fly line | R66C08-Gal4 | (Owald et al., 2015) | RRID:BDSC_49412 | |
Fly line | VT1211-Gal4 | (Owald et al., 2015) | VDRC: 202324 | |
Fly line | VT999036-Gal4 | (Aso et al., 2014a) | NA | Gift from Yoshinori Aso |
Fly line | MB320C-SplitGAL4 | (Aso et al., 2014a) | RRID:BDSC_68253 | |
Fly line | MB058B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68278 | |
Fly line | MB087C-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68366 | |
Fly line | MB296B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68308 | |
Fly line | MB301B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68311 | |
Fly line | MB630B-SplitGal4 | (Aso et al., 2014a) | RRID:BDSC_68334 | |
Fly line | *UAS-DAMBi | (Pimentel et al., 2016) | VDRC: 105324; RRID:FlyBase_FBst0478846 | |
Fly line | *UAS-GADi | (Barnstedt et al., 2016) | VDRC: 32344; RRID:FlyBase_FBst0459538 | |
Fly line | *UAS-Rdli | (Cheung and Scott, 2017) | VDRC: 41103; RRID:FlyBase_FBst0463935 | |
Fly line | UAS-5HT1Ai | (Lee et al., 2011) | VDRC: 106094; RRID:FlyBase_FBst0472248 | |
Fly line | *UAS-5HT1Bi | (Mohammad et al., 2016) | VDRC: 109929; RRID:FlyBase_FBst0476393 | |
Fly line | *UAS-5HT2Ai | (Mohammad et al., 2016) | VDRC: 102105; RRID:FlyBase_FBst0475582 | |
Fly line | UAS-5HT2Bi | (Mohammad et al., 2016) | VDRC: 102356; RRID:FlyBase_FBst0478281 | |
Fly line | UAS-5HT7i | (Dietzl et al., 2007) | VDRC: 104804; RRID:FlyBase_FBst0472736 | |
Fly line | *UAS-NPFRi | (Ni et al., 2009) | RRID:BDSC_25939 | |
Fly line | *UAS-sNPFRi | (Hong et al., 2012) | VDRC: 38925; RRID:FlyBase_FBst0462758 | |
Fly line | *UAS-dInRi | (Loh et al., 2017) | RRID:BDSC_35251 | |
Fly line | *UAS-DAR1i | (Yamagata et al., 2016) | NA | Gift from Hiromu Tanimoto |
Fly line | *UAS-DAMBi2 | (Hattori et al., 2017) | RRID:BDSC_51423 | |
Fly line | *UAS-GADi2 | (Koganezawa et al., 2016) | RRID:BDSC_51794 | |
Fly line | *UAS-Rdli2 | (Koganezawa et al., 2016) | RRID:BDSC_52903 | |
Fly line | *UAS-5HT1Bi2 | (Kaneko et al., 2017) | RRID:BDSC_33418 | |
Fly line | *UAS-5HT2A2 | (Ni et al., 2009) | RRID:BDSC_56870 | |
Fly line | *UAS-NPFRi2 | (Dietzl et al., 2007) | VDRC: 107663; RRID:FlyBase_FBst0481454 | |
Fly line | *UAS-dInRi2 | (Ni et al., 2009) | RRID:BDSC_51518 | |
Fly line | *UAS-sNPFRi2 | (Hu et al., 2017) | RRID:BDSC_27507 | |
Fly line | *UAS-DAR1i2 | (Yamagata et al., 2016) | NA | Gift from Hiromu Tanimoto |
Fly line | Orco2 | (Larsson et al., 2004) | RRID:BDSC_23130 | |
Fly line | IR25a2 | (Abuin et al., 2011) | RRID:BDSC_41737 | |
Fly line | IR8a1 | (Abuin et al., 2011) | RRID:BDSC_41744 | |
Fly line | DAMB | (Selcho et al., 2009) | Flybase: FBab0047678 | |
Fly line | DopR1 (dumb2) | (Qin et al., 2012) | Exelixis: f02676; RRID:FlyBase_FBst1017920 | |
Fly line | D2Rf06521 | (Marella et al., 2012) | Exelixis: f06521; RRID:FlyBase_FBst1020637 | |
Fly line | DopEcRc02142 | (Inagaki et al., 2012) | Exelixis: c02142; RRID:FlyBase_FBst1006135 | |
Fly line | UAS-TrpA1 | (Hamada et al., 2008) | Flybase: FBtp0040248 | Gift from Scott Waddell |
Fly line | UAS-shits1 | (Kitamoto, 2001) | Flybase: FBtp0013545 | Gift from Scott Waddell |
Fly line | UAS-mCD8-GFP | (Lee and Luo, 1999) | Flybase: FBtp0002652 | Gift from Scott Waddell |
Fly line | UAS-GCaMP6m | (Chen et al., 2013) | RRID:BDSC_42748 | |
Fly line | UAS-DenMark,UAS-Dsyd-1::GFP | (Owald et al., 2015) | NA | Gift from Scott Waddell |
Fly line | nSyb-GAL4 | (Pauli et al., 2008) | Flybase: FBtp0041245 | Gift from Scott Waddell |
Fly line | da-GAL4 | (Wang et al., 2017) | RRID:BDSC_55851 | |
Antibody | Mouse anti-brp (nc82) | Developmental Studys Hybridoma Bank (DSHB), IA, USA | RRID:AB_2314866 | |
Antibody | Chicken anti-GFP | Abcam, UK | RRID:AB_300798 | 1:5000 |
Antibody | Rabbit anti-Dsred | Clontech, CA, USA | RRID:AB_10013483 | 1:500 |
Antibody | Rat anti-mCD8α | Thermo Fisher Scientific, MA, USA | RRID:AB_10392843 | 1:100 |
Antibody | Goat anti-rabbit (Cy3) | Jackson ImmunoResearch, PA, USA | RRID:AB_2338006 | 1:400 |
Antibody | Goat anti-mouse (Cy3) | Jackson ImmunoResearch, PA, USA | RRID:AB_2338692 | 1:400 |
Antibody | Donkey anti-chicken (Alexa 488) | Jackson ImmunoResearch, PA, USA | RRID:AB_2340375 | 1:400 |
Antibody | Goat anti-rat (Alexa 488) | Thermo Fisher Scientific, MA, USA | RRID:AB_141373 | 1:400 |
Food odor | Dry yeast | Ferminpan red, Italy | NA | |
Food odor | Apple cider vinegar | Alce Nero, Italy | NA | |
Food odor | Banana powder | Gen Asia Biotech, Taiwan | NA | |
Chemical | Sucrose | Merck, Germany | Cat# 107687 | |
Chemical | Agar | BD, NJ, USA | Cat# 214530 | |
Chemical | Formaldehyde | Sigma, MO, USA | Cat# F8775 | |
Chemical | PBS | Sigma, MO, USA | Cat# P4417 | |
Chemical | Gold Antifade reagent | Thermo Fisher Scientific, MA, USA | Cat# S36937 | |
Chemical | paraffin wax | Sigma, MO, USA | Cat# 327304 | |
Serum | Normal goat serum | Jackson ImmunoResearch, PA, USA | RRID:AB_2336990 | |
Kits | TRIzol RNA Isolation reagents | Thermo Fisher Scientific, MA, USA | Cat# 15596026 | |
Kits | SuperScript IV First-Strand Synthesis System for RT-PCR Kit | Thermo Fisher Scientific, MA, USA | Cat# 18091050 | |
Software | Prism 7 | GraphPad, CA, USA | RRID:SCR_002798 | |
Software | MATLAB 2017a | MathWorks, MA, USA | RRID:SCR_001622 | |
Software | Fiji/ImageJ | Fiji | RRID:SCR_002285 |
*Asterisks indicate the RNAi lines whose knockdown efficiencies are shown in Figure 6—figure supplement 3.