Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Synthetic anti-IDE antibody Fab fragment (FabH11-E) | This study | NA | About 5 mg/ml for crystallization, and 0.15 mg/ml for cryoEM. |
| Antibody | Synthetic anti-IDE antibody Fab fragment Fab1Fab1 | (McCord et al., 2013) | NA | About 5 mg/ml for crystallization. |
| Recombinant protein (Human) | Cysteine-free IDE | This study | NA | Described as above. |
| Recombinant protein (Human) | Cysteine-free IDE-E111Q | This study | NA | Described as above. |
| Recombinant protein (Human) | Insulin | Sigma-Aldrich | 234-279-7 | |
| Recombinant protein (Human) | Amyloid β | (King et al., 2014) | NA | |
| Recombinant protein (Human) | Pepsin | Sigma-Aldrich | P6887-1G | |
| Chemical compound, drug | BDM44768 | (Deprez-Poulain et al., 2015a) | NA | |
| Chemical compound, drug | 6bK | (Maianti et al., 2014) | NA | |
| Chemical compound | D2O | Cambridge Isotope Laboratories, Inc. | DLM-4–1L | |
| Software, algorithm | Leginon 3.3 | (Suloway et al., 2005) | NA | http://emg.nysbc.org/redmine/projects/leginon/wiki/Leginon_Homepage |
| Software, algorithm | DoGpicker | (Voss et al., 2009) | NA | http://emg.nysbc.org/redmine/projects/software/wiki/DoGpicker |
| Software, algorithm | MotionCor2 | (Zheng et al., 2017) | NA | http://msg.ucsf.edu/em/software/motioncor2.html |
| Software, algorithm | Relion2.0 | (Scheres, 2012) | NA | http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page |
| Software, algorithm | Relion2.1 | (Scheres, 2012) | NA | http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page |
| Software, algorithm | 3DFSC | (Tan et al., 2017) | NA | https://github.com/nysbc/Anisotropy |
| Software, algorithm | UCSF ChimeraX | (Goddard et al., 2018) | NA | https://www.cgl.ucsf.edu/chimerax/ |
| Software, algorithm | UCSF Chimera | (Pettersen et al., 2004) | NA | https://www.cgl.ucsf.edu/chimera |
| Software, algorithm | PHENIX | (Adams et al., 2010) | NA | https://www.phenix-online.org/ |
| Software, algorithm | Phaser-MR | (McCoy et al., 2007) | NA | https://www.phenix-online.org/documentation/tutorials/mr.html |
| Software, algorithm | COOT | (Emsley et al., 2010a) | NA | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot |
| Software, algorithm | MolProbity | (Chen et al., 2010) | NA | http://www.ks.uiuc.edu/Research/mdff/ |
| Software, algorithm | PRIMUS | (Konarev et al., 2003) | NA | https://www.embl-hamburg.de/biosaxs/primus.html |
| Software, algorithm | CRYSOL | (Svergun et al., 1995) | NA | https://www.embl-hamburg.de/biosaxs/crysol.html |
| Software, algorithm | GNOM | (Svergun, 1992) | NA | https://www.embl-hamburg.de/biosaxs/gnom.html |
| Software, algorithm | SEQUEST/Proteome Discoverer Software | ThermoFisher Scientific | NA | https://www.thermofisher.com/order/catalog/product/OPTON-30795 |
| Software, algorithm | HDEXaminer | Sierra Analytics | NA | http://massspec.com/hdexaminer/ |