Figure 2.

IT pump infusion of recombinant adeno-associated virus serotype 9 (rAAV9) in mouse transduced spinal cord. (A) In vivo imaging showing the firefly luciferase expression from a single-stranded rAAV9.CB6-Fluc vector delivered by either a single-bolus IT injection of 5 × 10¹⁰ GC in 5 μL (bolus) or IT pump infusion of 2 × 10¹² GC in 200 μL (pump). (B) Epi-fluorescence imaging of native EGFP signal from a self-complementary (sc) AAV9.CB6-EGFP vector. Brain and spinal cord were dissected from mice receiving either a single-bolus IT injection of 5 × 10¹⁰ GC in 5 μL (bolus) or IT pump infusion of 2 × 10¹² GC in 200 μL (pump). (C) Immunofluorescence staining of mouse spinal cord tissue cryosections. Tail vein: injection of 2 × 10¹² GC of scAAV9.CB6-EGFP through tail vein in an adult mouse. Pump: IT pump infusion of the same vector of the same dose. Yellow arrows: co-localization of EGFP and NeuN (a neuronal cell marker) staining signals indicates transduction of neurons in the ventral horns. White arrows: lack of co-localization. Scale bar: 50 μm.