Figure 4.

The high EGFP expression in spinal cord resulting from IT pump infusion caused microgliosis. Native EGFP and anti-IBA1 immunostaining signals from spinal cord tissue sections. Mice were treated with either (A) 1 × 10¹¹ GC of scAAV9.CB6-EGFP (scAAV9.EGFP) or (B) 1 × 10¹¹ viral particles of empty rAAV9 vectors without a packaged genome (rAAV9.empty). Arrowhead indicates the increased IBA1 immunostaining signal that corresponds to the region with strong EGFP expression in the lumbar region.