Figure 5.

Comparing intracerebral ventricular (ICV) bolus injection and pump infusion of scAAV9.CB6-EGFP. (A) The immunostaining or native EGFP signals of mouse brain sections following either an ICV bolus injection of 2 × 10¹⁰ GC (bolus), or ICV pump infusion of 1 × 10¹¹ GC (pump). (B) Immunostaining of EGFP and NeuN of brain sections following ICV pump infusion. Co-localization of the signals indicates transduction of neurons in the hippocampus. Scale bar: 100 μm. (C) Native EGFP signal and BF images of cryosections of the liver, heart, and TA muscle. Scale bar: 50 μm.