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. 2018 Apr 1;29(2):75–85. doi: 10.1089/hgtb.2017.250

Figure 5.

Figure 5.

Comparing intracerebral ventricular (ICV) bolus injection and pump infusion of scAAV9.CB6-EGFP. (A) The immunostaining or native EGFP signals of mouse brain sections following either an ICV bolus injection of 2 × 1010 GC (bolus), or ICV pump infusion of 1 × 1011 GC (pump). (B) Immunostaining of EGFP and NeuN of brain sections following ICV pump infusion. Co-localization of the signals indicates transduction of neurons in the hippocampus. Scale bar: 100 μm. (C) Native EGFP signal and BF images of cryosections of the liver, heart, and TA muscle. Scale bar: 50 μm.