Figure 1.

Expression of ETR1 and ERS1 in yeast. A, Structure of ETR1 and ERS1. Hydrophobic, GAF, His kinase, and response regulator domains are indicated. Regions used for generating domain-specific antibodies are also indicated. B, Western blot with ERS1 antibody. Membrane fractions were isolated from yeast expressing ETR1, ERS1, or transformed with vector alone. Samples were incubated in the absence (−) or presence (+) of 100 mm dithiothreitol (DTT) for 1 h at 37°C, then subjected to SDS-PAGE. Positions of the ERS1 monomer and dimer are indicated. C, Western blot with ETR1 antibody. The same membrane fractions used in B were probed with the ETR1-HRR antibody. Positions of the ETR1 monomer and dimer are indicated.