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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Matrix Biol. 2017 Dec 6;67:107–122. doi: 10.1016/j.matbio.2017.11.012

Figure 3. Heparin increases matrix at early stages of assembly.

Figure 3

(A) CHO-677 cells were treated as in Figure 1 and analyzed at 1, 2, 4, 12, and 24 h after addition of FN and heparin followed by immunostaining for FN and fluorescence microscopy to visualize the matrix. Mean fluorescence fold change ± SE at each time point relative to No Hep at the same time is noted on the images. Scale bar indicates 50 μm. (n = 3, *p < 0.05). (B) Plot indicates the mean fluorescence intensity ± SE as a function of time for the No Hep and + Hep conditions (n=3).