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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Matrix Biol. 2017 Dec 6;67:107–122. doi: 10.1016/j.matbio.2017.11.012

Figure 4. Increased DOC-soluble FN at early times of treatment.

Figure 4

(A) Cells were treated as in Figure 1 legend. The DOC-soluble and insoluble matrix fractions of CHO-677 cells were immunoblotted after treatment for 4 h. An immunoblot of DOC-soluble material with anti-GAPDH antibodies was used as a loading control. (B) The DOC-soluble FN matrix fractions of CHO-677 cells were immunoblotted after 1 h or 2 h with FN plus or minus heparin. The mean fold change ± SE relative to no heparin at each time point, normalized to GAPDH, was calculated from 3 independent experiments. A representative blot is shown.