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. Author manuscript; available in PMC: 2019 Apr 19.
Published in final edited form as: Cell Chem Biol. 2018 Feb 1;25(4):392–402.e14. doi: 10.1016/j.chembiol.2018.01.006

Figure 4. Effect of CD1b point mutations on SGL antigen recognition.

Figure 4

(A) Location of CD1b point mutations in stably transfected C1R cells. The figure is based on PDB entry 1GZP (Gadola et al., 2002). Substitutions at residues colored green did not show an effect on A05 T-cell activation, while residues colored yellow showed moderate inhibition, and residues colored red showed complete inhibition (B) Expression of CD1b by each of the transfected C1R cells. Isotype indicates staining with an isotype control antibody. (C) IFN-γ production by A05 and A01 T-cell clone in the presence of AM Ac2SGL and transfected C1R cells. WT = wild type. Controls are shown in as white bars and CD1b mutants are shown as black bars. Data are representative of three independent experiments with triplicate wells. Error bars represent SEM of triplicate wells.