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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Matrix Biol. 2017 Dec 14;67:90–106. doi: 10.1016/j.matbio.2017.12.003

Figure 1. Substratum directs activated fibroblast phenotypic heterogeneity.

Figure 1

QRT-PCR (A) and representative flow cytometric analysis (B) of FAP and αSMA expression in fibroblasts cultured in 10% serum on tissue culture-treated plastic in the presence or absence of 75 μg/ml ascorbic acid (Vit. C) for 4 days. Data were compiled from 4 independent experiments and bar graphs depict the mean +/− SEM. (C) Collagen levels (as measured via hydroxyproline content) in FDMs deposited by fibroblasts in the presence or absence of 75 μg/ml Vit. C. Data were compiled from 2 independent experiments and bar graphs depict the mean +/− SEM. (D) Representative IF staining of FN and two-photon second harmonic generation imaging of fibrillar collagen in lung FDMs. QRT-PCR (E) and representative flow cytometric analysis (F) of FAP and αSMA expression in fibroblasts in 10% serum on tissue culture-treated plastic or FDM for 4 days. Data were compiled from 4 independent experiments and bar graphs depict the mean +/− SEM.