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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Matrix Biol. 2017 Dec 14;67:90–106. doi: 10.1016/j.matbio.2017.12.003

Figure 2. ECM composition and elasticity govern activated fibroblast phenotypic heterogeneity.

Figure 2

Representative phalloidin staining of the actin cytoskeleton (A) and Fap and Acta2 gene expression (B) in fibroblasts cultured in 10% serum on 2 versus 20 kPa FN- or COL I-coated hydrogels for 72 hours. Data were compiled from 4 independent experiments and bar graphs depict the mean +/− SEM. (C) Representative flow cytometric analysis, including quantification of relative median fluorescent intensities (MFI) for FAP and αSMA expression in fibroblasts cultured in 10% serum on 2 kPa (blue) versus 20 kPa (red) FN-coated hydrogels for 72 hours. Data were compiled from 3 independent experiments and bar graphs depict the mean +/− SEM. (D) QRT-PCR (top) and flow cytometric analysis (bottom) of FAP and αSMA expression in fibroblasts cultured in 10% serum on 2, 5, 12, and 20 kPa FN-coated hydrogels for 72 hours. Data were compiled from 2 independent experiments and bar graphs depict the mean +/− SEM. (E) Representative flow cytometric analysis, including quantification of relative MFI for FAP and αSMA expression in lung fibroblasts cultured in 10% serum on 2 kPa (blue) versus 20 kPa (red) COL I-coated hydrogels for 72 hours. Data were compiled from 3 independent experiments and bar graphs depict the mean +/− SEM.