QRT-PCR (A) and flow cytometric analysis (B) of FAP and αSMA expression in fibroblasts that were seeded in 1% serum on 2 versus 20 kPa COL I- or FN-coated hydrogels, allowed to adhere overnight, and subsequently treated with 2 ng/ml TGF-β for 48 (A) or 72 hours (B). Data were compiled from 4 independent experiments and bar graphs depict the mean +/− SEM. (C) QRT-PCR analysis of Fap, Acta2, and Cyp24a1 gene expression in fibroblasts that were seeded in 10% serum on FN-coated hydrogels (2 or 20 kPa), allowed to adhere overnight, and subsequently treated with calcipotriol (Cal; 100 or 500 nM) or vehicle control for 48 hours. Data were compiled from 3 independent experiments and bar graphs depict the mean +/− SEM.